Drf1-dependent kinase interacts with claspin through a conserved protein motif*

HIGHLIGHTS

• who: Daniel A. Gold from the EXPERIMENTAL PROCEDURES Xenopus Egg Extracts-Interphase egg extracts were treated with , ␮g/ml annealed (dA)70-(dT), (pA-pT) to activate checkpoint responses as described previously (20)To produce chromatin containing DNA replication blocks, the extracts were incubated with demembranated sperm nuclei (, , ␮l) and , ␮g/ml aphidicolin. Antibodies-Immunodepletion and immunoblotting of Claspin were performed with antibodies raised against residues, of Claspin (38). The anti-Claspin immunoprecipitations (IPs) in this paper were carried out with antibodies generated against residues, of Claspin (20). Anti-Drf1, antiCdc7, anti-Plx1, anti-replication protein A . . .
  

   

  

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