HIGHLIGHTS
- who: Stavrini Papadaki from the SchoolWestlake University have published the paper: Dual-expression system for blue fluorescent protein optimization, in the Journal: Scientific Reports Scientific Reports
- what: To streamline optimization of intracellular brightness of FPs using directed molecular evolution, the authors aimed to append high-throughput screening of large gene libraries expressed in E.coli with a rapid assessment of intracellular brightness of selected clones in mammalian cells. The authors developed two new BFPs, named Electra1 and Electra2, characterized by intracellular brightness and photostability suitable for multicolor neuroimaging in combination with other conventional FPs.
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