Dynamic changes in protein-protein interaction and protein phosphorylation probed with amine-reactive isotope tag*s

HIGHLIGHTS

• who: Marcus B. Smolka from the Ludwig InstituteUniversity of have published the Article: Dynamic Changes in Protein-Protein Interaction and Protein Phosphorylation Probed with Amine-reactive Isotope Tag*S, in the Journal: (JOURNAL) of April/25,/2005

SUMMARY

For post-purification labeling of protein samples, several aminereactive stable isotope-labeling reagents were developed, which led to perturbations in both the ionization efficiency and CID of the labeled peptides. In contrast, the abundance of 12 other phosphopeptides of Rad53 increased from 3- to 60-fold after MMS treatment. Three phosphopeptides of Yta7 were identified . . .

 



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