HIGHLIGHTS
- who: Kazuto Yoshimi from the (UNIVERSITY) have published the research work: Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3, in the Journal: (JOURNAL) of 22/06/2021
- how: All data were analyzed using PeakScanner software (Thermo Fisher Scientific). During engagement the feedback control for the cantilever's oscillation 11 Observation by hs-AFM To observe the reaction intermediates of EcoCascade/crRNA and dsDNA before and after addition of EcoCas3 and/or ATP at high spatial resolution the authors used 3-aminopropyltriethoxysilane treated mica (APTES-mica) as described previously55.
SUMMARY
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