Early life vitamin d depletion and mechanical loading determine methylation changes in the

HIGHLIGHTS

• who: Nevena Krstic from the The murine model has been described in detail previously (Fig3) [24]. Four-week-old C BL/, (Charles River, Kent, UK) female mice were purchased and housed at the University of Sheffield Biological Services Unit and had access to food and water ad libitum. All mice were housed in a single room with a, h light/dark cycle at , u00b0C under UV-filtered fluorescent lighting. Female mice were fed a vitamin, supplemented diet (, units/kg) or vitamin, free diet based on AIN G (Research Diets Inc., USA) diet from , weeks of age. Mice . . .
  

   

  

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