HIGHLIGHTS
SUMMARY
Several in_vivo and in_vitro studies have highlighted that coffee consumption is able to increase the Bifidobacterium spp. concentration in the colonic stage, as opposed to that of Bacteroides and Clostridium spp., with well-known positive effects, implying that coffee intake may have a prebiotic impact. Despite the fecal inoculum method representing the most appropriate protocol to replicate in_vitro colonic digestion, an increasing number of studies have reported that the combination of bacterial enzymes, such as Pronase E and Viscozyme L, represents a suitable alternative to reproducing intestinal fermentation. The data revealed that HT-29 . . .
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