HIGHLIGHTS
SUMMARY
Damaged sperm cells are characterized by low plasma_membrane integrity, acrosome and mitochondrial damage, and DNA damage, reducing their viability and motility. In studies on L-cysteine supplementation for frozen sperm diluents, there were differences in the breed of cattle, the macroscopic and microscopic characteristics of fresh sperm, the primary diluent, and the dosage of L-cysteine administered. Abnormality (%), acrosome damage (%), acrosomal integrity (%), DNA damage (%), DNA integrity (%), malondialdehyde (MDA) content (nmol/mL), plasma_membrane integrity (%), pregnancy rate (%), progressive motility (%), sperm viability (%), and total motility (%) were some of the performance parameters evaluated to examine the effect of . . .

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