HIGHLIGHTS
SUMMARY
The potential significance of generating an in_vitro community that mimics the in_vivo community was also recognized. The ability to efficiently and rapidly identify microorganisms has in turn led to methodological improvements for culturing, both for single-strain cultures of difficult oral organisms and for generating more biologically relevant in_vitro microbiomes. Several efforts have been directed toward creating experimental models (microcosms) of the oral microbiome in_vitro. A fruitful approach uses host plaque to inoculate in_vitro cultures, with supplementation by pig mucin used to mimic salivary mucin. Since selective conditions are expected to influence the community . . .
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