HIGHLIGHTS
- who: Crystal A. Mariano from the Representative samples from each lung region were collected to qualitatively observe untreated original structures compared to elastase and collagenase treated tissuesSamples were fixed in, % neutral buffered formalin immediately after testing and submerged in, % ethyl alcohol. Samples were sent to the University of California Irvine Experimental Tissue Resource Center to be embedded in wax paraffin blocks and cut into two slices collected onto glass slides. The samples were axially oriented to visualize the axially aligned connective tissue fibers [41]. Sections were stained with Masson`s Trichrome, exhibiting collagen fibers in grey and . . .
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