HIGHLIGHTS
- who: Jiheng Tian and colleagues from the (UNIVERSITY) have published the research work: Efficient Large-Scale and Scarless Genome Engineering Enables the Construction and Screening of Bacillus subtilis Biofuel Overproducers, in the Journal: (JOURNAL)
- what: The authors develop an method for and in the which can delete up to 134.3 kb DNA fragments 3.5 times as long as the previous report with a positivity rate of 100%. In comparison, the approach shows great advantages. This study provided an efficient CRISPR-Cas9 genome editing method which facilitated B. subtilis genome simplification.
- how . . .
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