Efficient simultaneous double dna knock-in in murine embryonic stem cells by crispr/cas9 ribonucleoprotein-mediated circular plasmid targeting for generating gene-manipulated mice

HIGHLIGHTS

• who: Manabu Ozawa from the LaboratoryThe University Tokyo, Tokyo, u20118639, Japan have published the Article: Efficient simultaneous double DNA knock-in in murine embryonic stem cells by CRISPR/Cas9 ribonucleoprotein-mediated circular plasmid targeting for generating gene-manipulated mice, in the Journal: Scientific Reports Scientific Reports
• what: The authors aimed to develop a simple and highly efficient KI method in mouse ES cells to efficiently develop genetically manipulated mouse models. Similar findings were observed in this study, i.e., all ES cell clones that became drug-resistant could not be confirmed to be accurate KI . . .
  

   

  

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