Elastase-mediated release of heparan sulfate proteoglycans from pulmonary fibroblast cultures

HIGHLIGHTS

• who: Jo Ann Buczek-Thomas from the passage before the start of all experimentsElastase Treatment of Fibroblast Cultures-At the onset of the experiment, cell cultures were rinsed once with phosphate-buffered saline and twice with , mM sodium bicarbonate buffer, pH, .4. Cells were treated with , mM sodium bicarbonate buffer supplemented with or without porcine pancreatic elastase for , min at , °C as indicated. The elastase concentrations used were based on previous studies that established the range of elastase needed to induce proteolytic damage to the elastin matrix without causing cytotoxic effects (10, ). Similar results were obtained using . . .
  

   

  

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