Electrostatic anti-cd33-antibody-protamine nanocarriers as platform for a targeted treatment of acute myeloid leukemia

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SUMMARY

    The authors observed decreased growth of xenografttransplanted AML cells as well as prolongation of survival in patient-derived xenotransplant (PDX) mouse model of AML in_vivo. Detection of internalized vesicles in cells and vesicular nanocarrier by fluorescence microscopy For detection of internalized vesicles in cells, 60 nM αCD33-mAB-P/P complex was coupled to 600 nM Allstar negative control siRNA-Alexa488 for 2 h at RT. Mice were treated 3 times weekly intraperitoneally with the indicated agents such as PBS, αCD33-mAB-P/P-scr-siRNA (scr, scrambled), αCD33-mAB-P/P-DNMT3A-siRNA . . .

     

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