Elephant tp53-retrogene 9 induces transcription-independent apoptosis at the mitochondria

HIGHLIGHTS

• who: Aidan J. Preston from the Cells were transfected with either Lipofectamine , (Invitrogen) (T G), or electroporated with either the Nucleofector (Lonza) (SAOS-2) or with the Neon (Invitrogen) (, WT, TP53-/-, U OS WT and U OS TP53-/-)Cells were sorted through the University of Utah Cores Facility using the BD FACSAria II, BD Fortessa, Propel Labs Avalon, or they were sorted using the Sony SH S. On each machine, the cells were sorted to exclude dead cells, exclude doublets, then cells were sorted for red or green fluorescence into a, well plate. have published the research . . .
  

   

  

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