Elevated ganglioside gm2 activator (gm2a) in human brain tissue reduces neurite integrity and spontaneous neuronal activity

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• who: Yi-Chen Hsieh from the All work was performed following IRB review and approval through Partners/BWH IRB (, )Human brain material was obtained from, ) the neuropathology core facility at Massachusetts General Hospital, ) Rush University Medical Center, and, ) Albany Medical Center. Tris Buffered Saline (TBS: , mM TrisHCl, mM NaCl, pH, .4) brain extracts were prepared using the method previously described [11]. Briefly, the tissue was dissected to isolate the gray matter, which was homogenized in ice-cold TBS at a ratio of, :, tissue weight to buffer volume within a Dounce homogenizer. This suspension was then subject to . . .
  

   

  

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