Elevated ntcp expression by an ipsc-derived human hepatocyte maintenance medium enhances hbv infection in ntcp-reconstituted hepg2 cells

HIGHLIGHTS

• who: Xinlei Li from the Hepatocyte maintenance medium (HMM) (Y30051, TaKaRa Bio USA) was used to pretreat or for long-term culture of Hep, and HepG2-NTCP cellsPrimary hepatocyte maintenance medium (PMM) was prepared according to previous publications [27, ]. The NTCP antibody was generously given by Dr. Bruno Stieger (Department of Chemistry and Applied Biosciences, University of Zurich, Switzerland). HBc (B058601-1, Agilent) and HBs (MD-05-0186, RayBiotech) antibodies were used to stain HBV viral proteins for immunofluorescence assay. Cyclosporine A (30024, MilliporeSigma), a chemical that reportedly targets NTCP to inhibit HBV infection was used. HBV . . .
  

   

  

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