Emergence of vancomycin-resistant enterococcus faecium at an australian hospital: a whole genome sequencing analysis

HIGHLIGHTS

• who: Kelvin W. C. Leong from the Enterococcal isolates from patient samples were cultured in , mL thioglycollate broth (TM0935) (Thermo Fisher Scientific, Waltham, Massachusetts, USA) at the RHH and were analysed at the School of Medicine, University of Tasmania (UTAS)Cell pellets were harvested by centrifugation at, × g for , minutes. The pellets were initially resuspended in a mixture of , µL lysozyme (, mg/mL) (Muramidase, VWR Chemicals, Radnor, PA, USA) and , µL phosphate buffered saline (PBS) and the suspension was incubated at , °C for , hour. Genomic DNA was extracted from , µL of lysate according to the DNeasy Blood . . .
  

   

  

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