Enamel defects and ameloblast-specific expression in enam knock-out/lacz knock-in mice*

HIGHLIGHTS

• who: Jan C.-C. Hu from the EXPERIMENTAL PROCEDURES Animal Protocol-All procedures involving animals were reviewed and approved by the University Committee on Use and Care of Animals at the University of MichiganKnock-in Targeting Construct-Construction of the targeting vector started with pLOz (Ozgene Pty. Ltd [, ]. Murdoch, Western Australia), a modified pBlueScript SK vector containing a lacZ (␤-galacatosidase) reporter with a mouse nuclear localization signal (NLS) upstream of a phosphoglycerine kinase (PGK) promoter driving a neomycin (Neo) selection marker, all inserted into the unique XbaI site in the multiple cloning region of the vector. The . . .
  

   

  

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