Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level

HIGHLIGHTS

• who: Fei Wang from the twice and resuspended in , u00b5L PBS +, % FBSPAECs were stained with , (, Human Alpha-Smooth muscle actin, RandD, Cat#IC G, :, dilution), (Porcine CD31/, AF700, R and D, #FAB, UG, :, dilution), and , (, Mouse Anti-Human CD45, BD Bioscience, #563879, :, dilution). HUVECs were stained with , (FITC Mouse Anti-Human , (BD Bioscience, #555445), :, dilution) and , (, Human alpha-Smooth muscle actin, R and D, Cat#IC G, :, dilution) separately. Cells were incubated on ice in the dark for , min. Before FC analysis, the stained cells were washed twice with pre-cooled PBS +, % FBS. At the final wash . . .
  

   

  

  If you want to have access to all the content you need to log in!

  Thanks :)

  Username or E-mail:

  If you don't have an account, you can create one here.