Engineering glucose-to-glycerol pathway in klebsiella pneumoniae and boosting 3-hydroxypropionic acid production through crispr interference

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  • who: Klebsiella pneumoniae et al. from the College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China, College of Bioscience and have published the research work: Engineering Glucose-to-Glycerol Pathway in Klebsiella pneumoniae and Boosting 3-Hydroxypropionic Acid Production Through CRISPR Interference, in the Journal: (JOURNAL)
  • how: The RT-qPCR data were analyzed using the u0394u0394CT method with 16S rRNA as an internal control. RTqPCR results showed that the transcription levels of gpd1 and gpp2 were significantly increased in KP-G12 compared to KPdCas9 . RT-qPCR results showed that the engineered . . .

     

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