HIGHLIGHTS
- who: Giselle C. Martu00edn-Hernu00e1ndez from the Germany), g/L (u00adNH4) , (u2265, %, Sigma-Aldrich, USA) and , g/L YNB without amino acids and ammonium sulphate (DifcoTM, Becton Dickinson, France). To inoculate the bioreactors, cells from a , u00b0C stock culture were streaked on YPD-agar (glucose , g/L, yeast extract , g/L, peptone , g/L) and incubated for , days. From the plates, cells were inoculated into , mL YPD in , mL baffled Erlenmeyer flasks. After incubation on an orbital shaker at , rpm and , u00b0C for , h, the cells were transferred to , mL-Falcon tubes, washed twice with NaCl-solution . . .
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