Enhanced production of d-pantothenic acid in corynebacterium glutamicum using an efficient crispr-cpf1 genome editing method

HIGHLIGHTS

• who: Rui Su from the College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China have published the research work: Enhanced production of d-pantothenic acid in Corynebacterium glutamicum using an efficient CRISPR-Cpf1 genome editing method, in the Journal: (JOURNAL)
• what: The authors compared the HR efficiencies of different recombinases including Redu03b1u03b2u03b3, RecET, RecA and RecET-RecA, and used kanamycin as a genetic marker, which enables rapid screening of genome-edited strains. The authors compared the IPTGinducible u00adPtac, arabinose-inducible u00adPBAD and propionate-inducible u00adPprpD2 in terms of FnCpf1 expression.
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