Enhancing the epidemiological surveillance of sars-cov-2 using sanger sequencing to identify circulating variants and recombinants

HIGHLIGHTS

• What: The authors identified circulating variants in samples collected in Belo Horizonte Brazil and detected a recombinant lineage using the Sanger method. For this, the authors compare Sanger and NGS sequencing in 227 samples (or 71,6% of the sequences). The study has some limitations, such as the difficulty in differentiating between Zeta, N.9, and Mu variants, which have the same mutation signature in the region analyzed with the primers used in the study. The authors propose that Sanger sequencing acts to (I) monitor the circulation of variants, complementing NGS with faster results, and_(II) assist . . .
  

   

  

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