Enzymatic synthesis of benzylisoquinoline alkaloids using a parallel cascade strategy and tyrosinase variants

HIGHLIGHTS

• who: Yu Wang from the online service u2018Clustal Omega` from EMBL-EBI (Cambridgeshire, UK)Alignment results were shown using the online service u2018Sequence Manipulation Suite` from the University of Alberta (Alberta, CA). Genes for the designed mutants were synthesised from Eurofins Scientific (Belgium). Random mutagenesis of CnTYR. Random mutagenesis was performed with E. coli XL, Red competent cells purchased from Agilent TechnologiesTM (Santa Clara, US). Mutation procedures followed the instruction manual. After mutation, plasmids were extracted using the Qiagen Mini Prep KitTM (Qiagen, Germany) and further transformed with E. coli , (DE3) competent cells. After cultivation at , u00b0C . . .
  

   

  

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