Epigenetically silenced apoptosis-associated tyrosine kinase (aatk) facilitates a decreased expression of cyclin d1 and wee1, phosphorylates tp53 and reduces cell proliferation in a kinase-dependent manner

HIGHLIGHTS

• who: Michelle L. Woods from the The cell lines TREx293, HEK T, and , were cultivated in DMEM or RPMI with, % FCS and, % Penicillin-Streptomycin at , u00b0C under, % CO , p53+/+ and , p53-/- were obtained from Thorsten Stiewe (University Marburg, Germany) and cultivated in DMEM [24]. The cells were transfected at a confluency of, % in serum-free media (Optimem, GIBCO) or at, cells (96-well plates) in serum-free media (Optimem, GIBCO) with, .16, or , u00b5g DNA (96-well, well, cm plates respectively). HEK T cells were transfected using PEI (Sigma). TREx, and , cells were transfected using Turbofect (Thermo . . .
  

   

  

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