Ercas12a and t5exo-ercas12a mediate simple and efficient genome editing in zebrafish

HIGHLIGHTS

  • who: Bingzhou Han and collaborators from the Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, Peking University University, Atlanta, GA, USA have published the article: ErCas12a and T5exo-ErCas12a Mediate Simple and Efficient Genome Editing in Zebrafish, in the Journal: Biology 2022, 11, 411. of 31/Dec/2021
  • what: The authors report efficient and heritable microhomology-mediated end joining (MMEJ) and non-homologous end joining (NHEJ)-based large fragment integration mediated by ErCas12a mRNA in zebrafish. The authors explored genome editing with ErCas12a in zebrafish for both mutagenic knockout and large . . .

     

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