Erianin suppresses constitutive activation of mapk signaling pathway by inhibition of craf and mek1/2

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• who: Penglei Wang from the , Western blotting analysis Protein was quantified using a BCA assay kit and then western blotting was conductedFor more details, refer to the previous paper [43]. Protein point mutations and purification To identify the binding sites, protein point mutations were constructed using the Fast Mutagenesis System (Cat#FM111, Trans, China). MAP , pET-28a (+), MAP , pET-28a (+), and CRAF pCDNA3., u00d7flag plasmids were obtained from Youbao Biotechnology Company. After sequence identification and IPTG (, mM) induction, the wild-type and mutant , and , proteins were purified with, u00d7His beads, and the wild-type and mutant CRAF . . .
  

   

  

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