Erk-mediated nelf-a phosphorylation promotes transcription elongation of immediate-early genes by releasing promoter-proximal pausing of rna polymerase ii

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• who: Seina Ohe from the Cell proliferation assay and mouse xenograft For the cell proliferation assay, u00d7 , HaCaT cells/well were seeded in triplicate in DMEM with, % FBS, and cell growth was monitored each day using the Cell Counting Kit, (Dojindo)For the mouse xenograft experiments, five-week-old female BALB/c-nude mice were purchased from Oriental Yeast (Tokyo, Japan)., u00d7, HaCaT-WT, HaCaT- A, RasHaCaT-WT, or Ras-HaCaT- A cells suspended in , u00b5l Matrigel (Corning) were injected subcutaneously into the left or right side flank of each mouse. Tumor volume (mm3) was measured with . . .
  

   

  

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