Establishment of a crispr/cas9-mediated efficient knockout system of trichoderma hamatum t21 and pigment synthesis pks gene knockout

HIGHLIGHTS

• who: Ning Luo and colleagues from the Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection have published the paper: Establishment of a CRISPR/Cas9-Mediated Efficient Knockout System of Trichoderma hamatum T21 and Pigment Synthesis PKS Gene Knockout, in the Journal: (JOURNAL)
• what: This is the main reason the genes of T. hamatum T21. In the study , the length of the homologous arm was designed to be 2000 bp, and each gene was targeted with two specific gRNAs, which significantly increased the efficiency of homologous recombination. The authors . . .
  

   

  

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