Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell rna sequencing and transient gene expression analysis

HIGHLIGHTS

• who: Ke Zhang from the State Key Laboratory of North China Crop Improvement and Regulation, College of Agronomy, Hebei Agricultural University, Baoding , have published the Article: Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis, in the Journal: (JOURNAL) of 15/Nov/2022
• what: The authors determined the suitable root age for protoplast isolation from taproots, defined the digestion time, and adjusted the preparation scheme to produce protoplasts that are usable for scRNA-seq in terms of cell number, viability, size, and purity. The . . .
  

   

  

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