Establishment of crfk cells for vaccine production by inactivating endogenous retrovirus with talen technology

HIGHLIGHTS

• who: Sayumi Shimode from the Genome Editing Innovation Center, Hiroshima University, Higashiu2011Hiroshima, Japan have published the Article: Establishment of CRFK cells for vaccine production by inactivating endogenous retrovirus with TALEN technology, in the Journal: Scientific Reports Scientific Reports of 21/03/2022
• what: To prevent the generation of infectious RD-114 virus by recombination, the authors aimed to knock out env genes of RDRS A2 and C1 using TALEN technology, which can destroy multiple targets.
• how: The authors chose a method to co-transfect with a puromycin-resistant gene plasmid for selection.
 



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