Evaluation and optimization of pcr primers for selective and quantitative detection of marine anme subclusters involved in sulfate-dependent anaerobic methane oxidation

HIGHLIGHTS

  • who: Peer H. A. Timmers from the Laboratory of Microbiology, Wageningen University, Stippeneng, WE Wageningen, the Netherlands have published the research: Evaluation and optimization of PCR primers for selective and quantitative detection of marine ANME subclusters involved in sulfate-dependent anaerobic methane oxidation, in the Journal: (JOURNAL)
  • what: As a result of this work the authors propose a standard workflow to facilitate selection of suitable for experiments on novel environmental samples. Since the environmental sample EB0 used in this study has a low amount of the ANME-2c subtype (Timmers et_al 2015a, b), this protocol . . .

     

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