Evidence for two crib domains in phospholipase d2 (pld2) that the enzyme uses to specifically bind to the small gtpase rac2*

HIGHLIGHTS

• who: Hong-Juan Peng from the CA)QIAquick gel extraction kit was purchase from Qiagen (Valencia, CA). The In-Fusion Ready BacPAK Vector Set and the In-Fusion Dry-Down PCR Cloning kit were from Clontech (Mountain View, CA). Tetramethyl benzidine (TMB) reagent (chromagen solution) was from Invitrogen. Ninety-six-well ELISA plates were from BD Biosciences. Precise protein gels were purchased from Fisher. Generation of PLD2-⌬CRIB Mutants-The construct pcDNA3.1-myc-PLD2aWT was used as a template to create to ⌬CRIB deletion mutants (⌬, and ⌬CRIB-2) after the QuikChangeXL site-directed mutagenesis protocol (Stratagene . . .
  

   

  

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