Evidence that endogenous formaldehyde produces immunogenic and atherogenic adduct epitopes

HIGHLIGHTS

• who: Jun Nakamura from the MaterialsNα-(tert-butoxycarbonyl)-L-Lys (Boc-Lys), ε-aminocaproic acid (6-ACA), AA, FA, BSA, trifluoroacetic acid, , ′, , ′-tetramethylbenzidine (TMB), human MPO, and H , were purchased from Sigma-Aldrich (St. Louis, MO). Malondialdehyde bis(dimethyl) acetal, Dynabeads , Amine, CarboxyLink Kit, the Imject EDC mcKLH Spin Kit, goat anti-rabbit IgG (H + L) antibody with HRP, goat anti-human IgG/IgM secondary antibody, and goat anti-mouse IgM secondary antibody were obtained from Thermo Scientific (Rockford, IL). Protein A column and autoLDL Cholesterol kit were purchased from GE Healthcare Life Sciences (Pittsburgh, PA) and Pointe . . .
  

   

  

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