Evolution of infectious bronchitis virus in the field after homologous vaccination introduction

HIGHLIGHTS

• who: Giovanni Franzo from the Four hundred and ten samples (pools of ten tracheal swabs), previously tested IBV positive using the VirusIBV-kit (Gensig, Southampton, UK), were collected between , and , from broiler farms located in northern Italy and delivered to the laboratory of infectious diseases of the DeptAnimal medicine, Production and Health (Padua University) for sequencing and genotyping. An IBV hypervariable region was amplified using the , and , primers as described by Cavanagh et al. [23] and Sanger sequenced in both directions using the same primers. All obtained sequences were first genotyped by comparison with the reference set . . .
  

   

  

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