Expansion of fgfr3-positive nucleus pulposus cells plays important roles in postnatal nucleus pulposus growth and regeneration

HIGHLIGHTS

• who: Meng Xu from the FGFR3-3*Flag-IRES-GFP (FGFR3-GFP) mice were generated by homologous recombination in embryonic stem cells targeting an IRES-GFP cassette to the, th exon of , locus (GFP allele)It was genotyped using primers mfgfr3-Tag-F (5`-AGCu200bCTGu200bAACu200bACAu200bGATu200bGGCu200b GTG-3`) and mfgfr3-Tag-R (5`-GCTu200bTGGu200bTCTu200bGTGu200bGGAu200b CTGu200b TTG-3`), which generated a , bp band showing the presence of the knock-in allele. Rosa26-mTmG mice (Stock No.023035), Rosa26-tdTomato mice (Stock No.007914), Rosa26-DTA mice (Stock No.006331) and Rosa26-Confetti (Stock NO.017492) were purchased from the . . .
  

   

  

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