HIGHLIGHTS
SUMMARY
As a simple disposal method, some works (Yushkevich et_al, 2006; Lein et_al, 2007) removed the damaged sections to alleviate the complexity of the subsequent serial section registration. The registration of the damaged section is not referred specifically to in commonly used serial section registration methods (Saalfeld et_al, 2012; Yoo et_al, 2017; Kajihara et_al, 2019). SEAMLeSS (Mitchell et_al, 2019; Macrina et_al, 2021) broke the smoothness constraint at cracks or folds to simulate discontinuous deformation. As another methodology, ssEM has been used in many large-volume reconstruction projects (Hildebrand et_al, 2017; Zheng et_al, 2018; Macrina et_al . . .
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