HIGHLIGHTS
SUMMARY
As GSE173955 only provided fasta files, the raw fasta files of GSE173955 need to be converted into gene_expression values in this study. Fourthly, "StringTie" was used to assemble the transcripts, estimate their abundances, and output the results in a gene_expression matrix. By incorporating these covariates into the model, the authors were able to adjust for their effects on the gene_expression data.After fitting the model, the authors calculated the empirical Bayes moderated t-statistics and p-values for each gene using the "eBayes" function in Limma. The development and increased use of NGS have . . .
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