Expression and secondary structure determination by nmr methods of the major house dust mite allergen der p 2*

HIGHLIGHTS

• who: Geoffrey A. Mueller from the lactopyranoside when the culture , reached, and the cells were harvested after, ., h. Isotopically enriched rDer p , (D S) was prepared by growing the bacteria in , minimal media with , glucose (, g/liter) and Nammonium sulfate (, g/liter) as the sole carbon and nitrogen sources. Selectively labeled samples of rDer p , (D S) were prepared using the E. coli trans-aminase-deficient strain DL-39. This strain was transfected with , phage to supply endogenous , polymerase for expression of rDer p , (D S). The , media was supplemented with , mg/liter of, glycine in combination . . .
  

   

  

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