Expression of osteopontin-5 splice variant in the mouse primary and metastatic breast cancer cells

HIGHLIGHTS

• who: Mohammad Kamalabadi-Farahani from the Induction of mammary tumor in BALB/c mice and isolation of primary and metastatic tumor cells, were performed as described in our previous work [19, ]The Ethics Committee of Shahroud University of Medical Sciences approved this study for ethics in animal research (registration number: IR.SHMU.REC.1400.265). The isolated cells were cultured in a DMEM with, % FBS, U/ml Penicillin, and , ug/ml Streptomycin (all from Gibco, USA). Ultimately, the cells were incubated at , u00b0C in, % u00ad, and passaged two times. Realu2011time qRTu2011PCR assay have published the Article: Expression . . .
  

   

  

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