HIGHLIGHTS
- who: Gonzalez et_al Reproductive Biology et al. from the Bouin's fixed human testicular specimens were dehydrated, embedded in paraffin and sectioned at , μmHematoxylin/eosin stain was used to analyze testicular morphology. The sections were deparaffinized with, % xylene and sequentially rehydrated with, , and, % ethanol. The slides were then blocked with, % hydrogen peroxide in absolute methanol for , min, washed twice with phosphate-buffered saline (PBS, pH, .4) for , min each time. The sections were subjected to saponine (0.5%) and proteinase K (, μg/ml) treatments. Nonspecific binding was blocked with normal serum blocking buffer for , min at room . . .
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