Extracellular calcium-sensing receptor is expressed in rat hepatocytes

HIGHLIGHTS

• who: Lucie Canaff from the Tissues-Protocols for obtaining rat tissues were approved by local animal ethics committeesTissues for RNA and Western blot analysis were flash frozen and stored at ⫺, °C. Reverse Transcription-Polymerase Chain Reaction Analysis of the CASR-Total RNA was extracted from tissues that were homogenized using a Polytron and from cells using TRIzol (Life Technologies, Inc.). RT-PCR was carried out as follows. First-strand cDNA was made by reverse transcribing , ␮g of DNase-I- treated total RNA with a recombinant superscript II RNase H (Life Technologies, Inc.) using oligo(dT)15, (Amersham . . .
  

   

  

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