Ezh2 suppression in glioblastoma shifts microglia toward m1 phenotype in tumor microenvironment

HIGHLIGHTS

• who: Yatao Yin from the gradient centrifugations as described previously [9]Blood samples were collected from healthy volunteers, and the procedure was approved by the ethics committee of Sun Yat-sen Memorial Hospital, Sun Yat-sen University. Monocytes were purified using , microbeads according to the manufacturer's directions (Miltenyi Biotec) and then were treated with , ng/ml macrophage colony stimulating factor (M-CSF, switching monocytes to , phenotype macrophages) and cultured at , °C and, % , in ultra-low attachment flasks (Corning) for , day in the RPMI , medium with, % fetal bovine serum (FBS, Gibco). Isolation and culture of microglia have . . .
  

   

  

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