Farnesyl pyrophosphate compartmentalization in the green microalga chlamydomonas reinhardtii during heterologous (e)-α-bisabolene production

HIGHLIGHTS

• who: Julian Wichmann from the Cloning was performed with Thermo Scientific FastDigest restriction enzymes and the Rapid DNA Dephos and Ligation Kit (Roche) according to manufacturers` protocols, high fidelity polymerase (New England Biolabs) was used to conduct PCRs following manufacturer`s protocols, with primers listed in Additional file, . All plasmid sequences were confirmed by sequencing (Sequencing Core Facility, CeBiTec, Bielefeld University, Germany). Plasmids were maintained in Escherichia coli DH5u03b1. Nucleotide sequences (AgBS (UniProt ID:, ) [8], CrFPPS (Uniprot ID: A IX41), Saccharomyces cerevisiae , (UniProt ID:, ) [7] were codon optimized by back translating the amino acid sequence and synthesized . . .
  

   

  

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