HIGHLIGHTS
SUMMARY
An inducible RelA+ expression cassette further allows to precisely set the basal level of ppGpp, and thus nominal growth rate, in any bacterial strain. In fact, co-existence of multiple strains in co-culture is contingent on tightly matching growth rates, wherein small growth rate differences between the strains typically lead to extinction of the slower growing strain18-20. To mitigate the consequences of gene_expression burden, researchers have devised methods that make synthetic gene_expression robust to changes in the availability of cellular resources21-25. The problem of controlling growth rate has been addressed by . . .
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