Fine-mapping the immunodominant antibody epitopes on consensus sequence-based hiv-1 envelope trimer vaccine candidates

HIGHLIGHTS

• who: E. I. M. M. Reiss from the exchanged into Tris-buffered saline (TBS) buffer (, mM Tris, mM NaCl, pH, ) using Vivaspin filters with a cut-off value of , kDa (Cytiva). TZM-bl Neutralization assays TZM-bl cell neutralization assays using Env-pseudotyped viruses were performed at three sites: the Amsterdam UMC (AUMC)22, Duke University (DU)43, and Ospedale San Raffaele(OSR)45. Neutralization by Abs and sera were measured as a function of reductions in luciferase (Luc) reporter gene expression after a single round of infection in TZM-bl cells43-45. At the AUMC, mAb . . .
  

   

  

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