First demonstration of the circulation of a pneumovirus in french pigs by detection of anti-swine orthopneumovirus nucleoprotein antibodies

HIGHLIGHTS

• who: Charles-Adrien Richard from the et alfound that, % of pigs sera from , herds in Northern Ireland were reactive with BRSV antigens [5]. Although SOV has not yet been isolated, the complete genomic sequence of SOV (strain, ) is available (GenBank accession number: KX364383.1). We thus used the published sequences to synthesize expression vectors for nucleoprotein (N) and phosphoprotein (P) in order to express these proteins in bacteria. As developed previously with HRSV [6], co-expression of the, terminal region of SOV P fused to GST together with SOV N allowed us to purify SOV N nanorings . . .
  

   

  

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