First record of trypanosoma evansi dna in dichelacera alcicornis and dichelacera januarii (diptera: tabanidae) flies in south america

HIGHLIGHTS

• who: Carlos Josu00e9 Raupp Ramos from the Flies were individually screened for the presence of Trypanosoma evansi and Trypanosoma vivax using specific oligonucleotide primers, RoTat , forward, u2032GCGu200bGGGu200b TGTu200bTTAu200bAAGu200bCAAu200bTA3u2032 and RoTat, ., reverse, u2032ATTu200b AGTu200bGCTu200bGCGu200bTGTu200bGTTu200bCG3u2032 for T. evansi [24], and Tvi, u2032GCTu200bCTCu200bCAAu200bTCTu200bTAAu200bCCCu200bTA3u2032 and Tvi, u2032GTTu200bCCAu200bGGCu200bGTGu200bCAAu200bACGu200bTC3u2032 for T. vivax [25]. The first two primers amplified a, base pair (bp) fragment and the second two a, bp fragment. Polymerase chain reaction (PCR) was conducted in a, u00b5L reaction mixture comprising, ., u00b5L deionized water, ., u00b5L u00adMgCl2,u00b5L Taq DNA polymerase, u00b5L deoxyribonucleotide triphosphate mix (, mM), u00b5L of, u00d7 Taq DNA polymerase buffer . . .
  

   

  

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