Formation of a distinctive complex between the inducible bacterial lysine decarboxylase and a novel aaa+ atpase*

HIGHLIGHTS

• who: Jamie Snider from the gctgtaataatttatcgccgccagcgcatatgaatatcctccttag-3⬘) to construct the viaA (yieM_ECOLI) deletion strain, cells were inoculated from single colonies into , ml of LB media and grown at , °C with shaking to , ⫽, ., , .8. , ml of culture was heated in a , °C water bath with shaking for , min to induce expression of the Lambda Red genes exo, bet, and gam. Cells were immediately cooled on ice for , min. Cells were made electrocompetent and then transformed with, ␮l of linear PCR product using a Bio-Rad GenePulser. Transformed cells were diluted into , ml of LB and grown at , °C . . .
  

   

  

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