HIGHLIGHTS
- who: Gordon S. Huggins from the , ), and the seventh and eighth zinc fingers (F7-, aa , )Full-length , cloned into the BamHI site of the pc, vector was used for expression in eukaryotic cells (9). pM-FOG-2(aa, )C S (M, ) and, C S (F, ) were made using site-directed polymerase chain reaction-based mutagenesis using synthetic oligonucleotide primers (Table I). pM-FOG-2(aa, )-C, C S (M, ) was made by cloning the, ⬘ HindIII fragment from pM-FOG2(aa, )-C S into pM-FOG-2(aa, )-C S. All constructs were sequenced using standard techniques. PACT2 . . .
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